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寡核苷酸是短的、單鏈或雙鏈的DNA或RNA分子。其合成方法發(fā)展歷程：從Michelson和Todd早期的H-磷酸鹽和磷酸三酯法（H-Phosphonate & Phosphotriester）；到1950年代Khorana的磷酸二酯法（Phosphodiester）；以及1960-1970年代的磷酸三酯法（Phosphotriester）和亞磷酸三酯法（Phosphite triester）的發(fā)展。而直至1980年代，Marvin Caruthers的亞磷酰胺法（Phosphoramidite），加上自動化固相技術(shù)，成為了目前主流的寡核苷酸合成技術(shù)方法。在聚合酶鏈反應(yīng)(PCR)、核酸測序、基因檢測、核酸藥物研發(fā)等領(lǐng)域有廣泛應(yīng)用。
亞磷酰胺寡聚體的合成在3'-5'方向進行(與生物合成中DNA復(fù)制的5'-3'方向相反)。每個合成周期添加一個核苷酸。亞磷酰胺DNA合成循環(huán)如圖所示。
亞磷酰胺DNA合成循環(huán)
保護核苷
| 產(chǎn)品號 |
中文名稱 |
英文名稱 |
級別 |
Cas |
規(guī)格 |
| I119475 |
N2-異丁酰基-2′-脫氧鳥苷 |
N2-Isobutyryl-2′-deoxyguanosine |
98% |
68892-42-2 |
100mg,250mg,1g,5g |
| I119519 |
5'-O-(4,4'-二甲氧基三苯基)-N2-異丁?；?2'-脫氧鳥苷 |
N2-Isobutyryl-5′-O-(4,4′-dimethoxytrityl)-2′-deoxyguanosine |
99.00% |
68892-41-1 |
1g,5g,10g,25g,100g |
| N159691 |
N6-二苯甲酰基腺苷-2',3'-二苯甲酸酯 |
N6-Dibenzoyladenosine 2',3'-Dibenzoate |
>98.0%(HPLC)(T) |
58463-04-0 |
100mg,1g |
| B119471 |
N4-苯甲酰基-5′-O-(4,4′-二甲氧基三苯基)-2′-脫氧胞苷 |
N4-Benzoyl-5′-O-(4,4′-dimethoxytrityl)-2′-deoxycytidine |
99% |
67219-55-0 |
1g,5g |
| B103100 |
N4-苯甲酰胞苷 |
N4-Benzoylcytidine |
98% |
13089-48-0 |
1g,5g,25g |
| B119532 |
N4-苯甲?；?2'-脫氧胞苷 |
N4-Benzoyl-2′-deoxycytidine |
98.00% |
4836-13-9 |
250mg,1g,5g |
| B122954 |
N6-苯甲?；?2'-脫氧腺苷水合物 |
N6-Benzoyl-2′-deoxyadenosine hydrate |
98% |
206752-42-3,4546-72-9 |
250mg,1g,5g |
| B119470 |
N6-苯甲?；?5′-O-(4,4′-二甲氧基三苯基)-2′-脫氧腺苷 |
N6-Benzoyl-5′-O-(4,4′-dimethoxytrityl)-2′-deoxyadenosine |
99% |
64325-78-6 |
100mg,1g,5g |
| O117684 |
5′-O-(4,4′-二甲氧基三苯甲基)胸苷 |
5′-O-(4,4′-Dimethoxytrityl)thymidine |
98.00% |
40615-39-2 |
1g,5g,25g |
| B119477 |
N6-苯甲?；佘?/td>
| N-Benzoyladenosine |
97% |
4546-55-8 |
1g,5g,25g |
核糖，2'-脫氧核糖
| 產(chǎn)品號 |
中文名稱 |
英文名稱 |
級別 |
Cas |
規(guī)格 |
| R105972 |
L-核糖 |
L-(+)-Ribose |
98% |
24259-59-4 |
1g,5g,10g,25g,100g |
| T107915 |
四乙酰核糖 |
1,2,3,5-Tetra-O-acetyl-β-D-ribofuranose |
98% |
13035-61-5 |
5g,25g,100g,500g |
| R104821 |
D-(-)-核糖 |
D-(-)-Ribose |
≥99%（HPLC) |
50-69-1 |
5g,25g,100g,250g,500g |
| R104820 |
D-(-)-核糖 |
D-(-)-Ribose |
98% |
50-69-1 |
100g,500g,2.5Kg |
| R103230 |
1-乙酰基-2,3,5-三苯甲酰氧基-1-beta-D-呋喃核糖 |
β-D-Ribofuranose 1-acetate 2,3,5-tribenzoate |
98% |
6974-32-9 |
1g,5g,25g,100g |
| M158368 |
甲基-β-D-呋喃核糖苷 |
Methyl β-D-Ribofuranoside |
>97.0%(HPLC) |
7473-45-2 |
1g,5g,25g,100g |
| D121077 |
3,5-二-O-(對甲苯酰)-2-脫氧-Α-D-呋喃核糖氯 |
3,5-Di-O-(p-toluyl)-2-deoxy-D-ribofuranosyl chloride |
90% |
4330-21-6 |
250mg |
| D104784 |
2-脫氧-D-核糖 |
2-Deoxy-D-Ribose |
98% |
533-67-5 |
1g,5g,25g,100g |
| D123989 |
2-脫氧-2,2-二氟-D-赤-戊糖酸γ-內(nèi)酯3,5-二苯甲酸酯 |
2-Deoxy-2,2-difluoro-D-erythro-pentonic Acid γ-Lactone 3,5-Dibenzoate |
98% |
122111-01-7 |
1g,5g,25g,100g |
| T139496 |
2-脫氧-2-氟-1,3,5-三-O-苯甲?；?α-D-阿拉伯呋喃糖 |
2-Deoxy-2-fluoro-1,3,5-tri-O-benzoyl-α-D- arabinofuranose |
- |
97614-43-2 |
1g,5g,25g |
硫轉(zhuǎn)移試劑
| 產(chǎn)品號 |
中文名稱 |
英文名稱 |
級別 |
Cas |
規(guī)格 |
| X162986 |
氫化黃原素 |
Xanthane Hydride |
>95.0%(HPLC) |
6846-35-1 |
5g,25g,100g,500g |
| H157193 |
3H-1,2-苯并二硫醇-3-酮1,1-二氧化物 |
3H-1,2-Benzodithiol-3-one 1,1-Dioxide |
>98.0%(HPLC) |
66304-01-6 |
250mg,1g,5g |
| B152485 |
雙(苯乙酰)二硫化物 |
Bis(phenylacetyl) Disulfide |
>98.0%(HPLC) |
15088-78-5 |
5g,25g |
磷酸化試劑，磷硫化試劑
| 產(chǎn)品號 |
中文名稱 |
英文名稱 |
級別 |
Cas |
規(guī)格 |
| M157887 |
二氯磷酸甲酯 |
Methyl Phosphorodichloridate |
>95.0%(T) |
677-24-7 |
5g,25g,100g |
| C119480 |
2-氰乙基N,N,N′,N′-四異丙基亞磷酰二胺 |
2-Cyanoethyl N,N,N′,N′-tetraisopropylphosphordiamidite |
97% |
102691-36-1 |
1g,5g,25g,100g |
| T162687 |
焦磷酸四芐酯 |
Tetrabenzyl Pyrophosphate |
>98.0%(HPLC) |
990-91-0 |
250mg,1g,5g,25g |
| P104277 |
二氯化磷酸苯酯 |
Phenyl dichlorophosphate |
98% |
770-12-7 |
25g,100g,500g |
| B152354 |
二(四丁銨)焦磷酸二氫鹽 |
Bis(tetrabutylammonium) Dihydrogen Pyrophosphate |
>96.0%(T) |
857447-79-1 |
50mg,200mg |
| H157193 |
3H-1,2-苯并二硫醇-3-酮1,1-二氧化物 |
3H-1,2-Benzodithiol-3-one 1,1-Dioxide |
>98.0%(HPLC) |
66304-01-6 |
250mg,1g,5g |
| C135875 |
2-氯-1,3,2-二氧磷雜環(huán)戊烷 2-氧化物 |
2-Chloro-1,3,2-dioxaphospholane-2-oxide |
≥95.0% |
6609-64-9 |
5g,25g,100g |
| D156020 |
氯硫代磷酸二乙酯 |
Diethyl Chlorothiophosphate |
>97.0%(GC) |
2524-04-1 |
5g,25g,100g |
| C154011 |
2-氯苯基二氯膦酸酯[磷酸化劑] |
2-Chlorophenyl Phosphorodichloridate [Phosphorylating Agent] |
>97.0%(T) |
15074-54-1 |
1g,5g,25g,100g |
| C154013 |
2-氯-4H-1,3,2-苯并二氧磷-4-酮 |
2-Chloro-4H-1,3,2-benzodioxaphosphorin-4-one |
>95.0% |
5381-99-7 |
1g,5g,25g |
| D109475 |
氯代磷酸二苯酯 |
Diphenyl phosphoryl chloride |
97% |
2524-64-3 |
25g,100g,500g |
| M157888 |
二氯亞磷酸甲酯[亞磷酸化劑] |
Methyl Dichlorophosphite [Phosphitylating Agent] |
>95.0%(T) |
3279-26-3 |
5g,10g,25g,100g |
| B152485 |
雙(苯乙酰)二硫化物 |
Bis(phenylacetyl) Disulfide |
>98.0%(HPLC) |
15088-78-5 |
5g,25g |
| C154012 |
4-氯苯基二氯磷酸酯 |
4-Chlorophenyl Phosphorodichloridate |
>97.0%(T) |
772-79-2 |
5g,25g,100g |
| D110276 |
焦磷酰氯 |
Diphosphoryl chloride |
97% |
13498-14-1 |
5g,25g,100g,500g |
| C153709 |
5-氯水楊基-N,N-二異丙基亞磷酰胺 |
5-Chlorosaligenyl-N,N-diisopropylphosphoramidite |
>95.0%(HPLC) |
1620086-77-2 |
200mg,1g |
| C154014 |
2-氯-1,3,2-二氧磷雜環(huán)戊烷 |
2-Chloro-1,3,2-dioxaphospholane |
>97.0%(GC) |
822-39-9 |
5g,25g,100g |
| D138186 |
N,N-二異丙基亞磷酰胺二叔丁酯 |
Di-tert-butyl N,N-diisopropylphosphoramidite |
≥95.0% |
137348-86-8 |
1g,5g |
| D122720 |
二芐基 N,N-二異丙基亞磷酰胺 |
Dibenzyl N,N-Diisopropylphosphoramidite |
98% |
108549-23-1 |
1g,5g,25g,100g |
縮合劑
| 產(chǎn)品號 |
中文名稱 |
英文名稱 |
級別 |
Cas |
規(guī)格 |
| N132858 |
3-硝基-1,2,4-三唑 |
3-Nitro-1,2,4-triazole |
≥98.0%(HPLC) |
24807-55-4 |
1g,5g,25g,100g |
| M121621 |
均三甲苯基磺酰氯 |
2-Mesitylenesulfonyl chloride |
99% |
773-64-8 |
5g,25g,100g,500g |
| C113337 |
N,N'-羰基二(1,2,4-三氮唑) |
1,1'-Carbonyl-di-(1,2,4-triazole) |
96% |
41864-22-6 |
5g,25g,100g |
| M135672 |
2,4-二磺酰氯基均三甲苯 |
2,4-Mesitylenedisulfonyl Dichloride |
≥98.0% |
68985-08-0 |
5g,25g |
| T109597 |
特戊酰氯 |
Trimethylacetyl chloride |
98% |
3282-30-2 |
25ml,100ml,500ml,2.5L,10L |
| B115197 |
5-芐硫基四氮唑 |
5-(Benzylthio)-1H-tetrazole |
98.50% |
21871-47-6 |
5g,25g,100g |
| D106074 |
N,N'-二環(huán)己基碳二亞胺(DCC) |
N,N′-Dicyclohexylcarbodiimide |
99.00% |
538-75-0 |
25g,100g,500g,1kg,2.5kg,10kg |
| C153419 |
1-(氰甲基)哌啶四氟硼酸鹽 |
1-(Cyanomethyl)piperidinium Tetrafluoroborate |
>98.0%(N) |
434937-12-9 |
1g,5g |
| T113353 |
1-(2,4,6-三異丙基苯基磺酰)咪唑 |
1-(2,4,6-Triisopropylbenzenesulfonyl)imidazole |
98% |
50257-40-4 |
1g,5g,10g,25g |
| D109319 |
4,5-二氰基咪唑 |
4,5-Dicyanoimidazole |
98% |
1122-28-7 |
5g,25g,100g |
| D109320 |
4,5-二氰基咪唑 |
4,5-Dicyanoimidazole |
99% |
1122-28-7 |
5g,25g,100g |
| T161612 |
2,4,5,6-四甲基苯二磺酰二氯 |
2,4,5,6-Tetramethylbenzenedisulfonyl Dichloride |
>98.0%(T) |
97997-76-7 |
1g,5g |
| T138965 |
1-(2,4,6-三異丙基苯磺酰)-1,2,4-三唑 |
1-(2,4,6-Triisopropylphenylsulfonyl)-1,2,4-triazole |
>98.0%(HPLC) |
54230-60-3 |
1g,5g,25g |
| T106183 |
1-對甲基苯磺酰咪唑 |
1-(p-Toluenesulfonyl)imidazole |
99% |
2232-08-8 |
5g,25g,100g,500g |
| T109341 |
2,4,6-三異丙基苯磺酰氯 |
2,4,6-Triisopropylbenzenesulfonyl chloride |
97% |
6553-96-4 |
5g,25g,100g,500g |
| D105651 |
N,N'-二環(huán)己基-4-嗎啉脒 |
N,N′-Dicyclohexyl-4-morpholinecarboxamidine |
98% |
4975-73-9 |
5g,25g,100g,500g |
| T100645 |
1,2,4-三氮唑 |
1,2,4-Triazole |
99% |
288-88-0 |
25g,100g,250g,500g |
| E111310 |
5-乙硫基四氮唑 |
5-(Ethylthio)-1H-tetrazole |
98% |
89797-68-2 |
1g,5g,25g,100g |
羥基保護劑，氨基保護劑
| 產(chǎn)品號 |
中文名稱 |
英文名稱 |
級別 |
Cas |
規(guī)格 |
| I107549 |
異丁酰氯 |
Isobutyryl Chloride |
98% |
79-30-1 |
25ml,100ml,500ml,2.5L |
| H106017 |
六甲基二硅胺烷 |
Hexamethyl disilylamine |
AR,98% |
999-97-3 |
100ml,500ml,2.5L,10L |
| D154192 |
二叔丁基二氯硅烷 |
Di-tert-butyldichlorosilane |
>95.0%(GC) |
18395-90-9 |
1g,5g |
| N106603 |
2-硝基溴化芐 |
2-Nitrobenzyl bromide |
97% |
3958-60-9 |
5g,25g,100g |
| P160117 |
苯氧基乙酰氯 |
Phenoxyacetyl Chloride |
>98.0%(GC) |
701-99-5 |
25g,100g,500g |
| B152896 |
1,3-苯并二硫代吡咯四氟化硼鹽[羥基保護劑] |
1,3-Benzodithiolylium Tetrafluoroborate [Hydroxyl-Protecting Agent] |
>96.0%(T) |
57842-27-0 |
1g,5g |
| T109041 |
N,O-雙(三甲基硅烷基)乙酰胺 |
N,O-Bis(trimethylsilyl)acetamide |
95% |
10416-59-8 |
25ml,100ml,500ml,5L |
| T131644 |
N,O-雙(三甲基硅烷基)乙酰胺 |
TMS-BA |
用于GC衍生化 |
10416-59-8 |
5ml,25ml |
| A108664 |
乙酰氯 |
Acetyl chloride |
99%,無色 |
75-36-5 |
500ml,2.5L |
| A108662 |
乙酰氯 |
Acetyl chloride |
AR,98% |
75-36-5 |
100ml,500ml,12*500ml,2.5L,10L |
| C134968 |
2-氯代三苯甲基氯 |
2-Chlorotrityl Chloride |
≥97.0% |
42074-68-0 |
25g,100g,500g,2.5kg |
| T109597 |
特戊酰氯 |
Trimethylacetyl chloride |
98% |
3282-30-2 |
25ml,100ml,500ml,2.5L,10L |
| D106289 |
N,N-二甲基甲酰胺二甲縮醛 |
DMF-DMA |
98%,用于酯化 |
4637-24-5 |
25g,100g,500g |
| D106288 |
N,N-二甲基甲酰胺二甲縮醛 |
N,N-Dimethylformamide Dimethyl Acetal |
97% |
4637-24-5 |
25ml,100ml,500ml |
| T106184 |
三苯基氯甲烷 |
Trityl Chloride |
97% |
76-83-5 |
25g,100g,250g,500g,2.5kg |
| C153889 |
4-氯苯氧基乙酰氯 |
4-Chlorophenoxyacetyl Chloride |
>98.0%(T) |
4122-68-3 |
5g,10g,25g,100g,500g |
| T109341 |
2,4,6-三異丙基苯磺酰氯 |
2,4,6-Triisopropylbenzenesulfonyl chloride |
97% |
6553-96-4 |
5g,25g,100g,500g |
| D109292 |
4,4'-二甲氧基三苯基氯甲烷 |
4,4′-Dimethoxytrityl chloride |
>97.0% |
40615-36-9 |
5g,25g,100g,500g |
| D119481 |
二叔丁基硅基雙(三氟甲烷磺酸) |
Di-tert-butylsilyl bis(trifluoromethanesulfonate) |
97% |
85272-31-7 |
1g,5g,25g |
| B111191 |
叔丁基二苯基氯硅烷 |
tert-Butyldiphenylchlorosilane |
98% |
58479-61-1 |
10g,25g,100g,250g |
| B103644 |
叔丁基二甲基氯硅烷 |
tert-Butyldimethylsilyl chloride |
97% |
18162-48-6 |
25g,100g,250g,500g |
| T162634 |
4,4',4''-三(苯酰氧基)三苯甲基溴[羥基保護劑] |
4,4',4''-Tris(benzoyloxy)trityl Bromide [Hydroxyl Protecting Agent] |
>97.0%(T) |
86610-66-4 |
1g,5g,25g |
| D113529 |
1,3-二氯四甲基二硅氧烷 |
1,3-Dichloro-1,1,3,3-tetramethyldisiloxane |
95% |
2401-73-2 |
5g,25g,100g |
| D139183 |
1,3二氯-1,1,3,3-四異丙基二硅氧烷 |
1,3-Dichloro-1,1,3,3-tetraisopropyldisiloxane |
≥97% |
69304-37-6 |
1g,5g,25g,100g |
| M100611 |
4-甲氧基苯甲酰氯 |
4-Methoxybenzoyl chloride |
97% |
100-07-2 |
25g,100g,500g |
| B104566 |
苯甲酰氯 |
Benzoyl chloride |
ACS, ≥99.0% (T) |
98-88-4 |
500g |
| C104814 |
三甲基氯硅烷(TMCS) |
Chlorotrimethylsilane |
≥99.0%(GC) |
75-77-4 |
100ml,250ml,500ml,2.5L,10L |
| C104813 |
三甲基氯硅烷(TMCS) |
Chlorotrimethylsilane |
>98.0%(GC) |
75-77-4 |
100ml,250ml,500ml,2.5L,10L |
| T106616 |
三氟甲磺酸三甲基硅酯 |
Trimethylsilyl trifluoromethanesulfonate |
98.00% |
27607-77-8 |
5g,10g,25g,50g,100g,250g,500g |
| M113503 |
4-甲氧基氯化三苯甲烷 |
4-Monomethoxytrityl chloride |
97% |
14470-28-1 |
5g,10g,25g,100g,250g |
| B104818 |
苯甲酸酐 |
Benzoic anhydride |
98% |
93-97-0 |
25g,100g,500g |
脫保護劑
| 產(chǎn)品號 |
中文名稱 |
英文名稱 |
級別 |
Cas |
規(guī)格 |
| T103291 |
三氟乙酸（TFA） |
Trifluoroacetic acid |
AR,99.0% |
76-05-1 |
100g,500g,2.5kg |
| H107501 |
六氟異丙醇 |
1,1,1,3,3,3-Hexafluoro-2-propanol |
99.50% |
920-66-1 |
25g,100g,250g,500g,2.5kg |
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